WebContact. New England Biolabs GmbH Brüningstr. 50; Geb. B852 D-65926 Frankfurt am Main Tel: +49-69/305-23140 E-Mail: [email protected] WebT7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase (1,2).
Modified Andolfatto- reduced rep sequencing v1 - ResearchGate
Web1. A method for obtaining sequence information from a nucleic acid, the method comprising the steps of: capturing target nucleic acids with a sequence-specific capture probe to produce a target/probe duplex; melting said target/probe duplex to release said target nucleic acids; annealing a primer to the target nucleic acids to produce a target/primer … WebNEB T4 DNA Ligase ( NEB #M0202) is the most extensively used ligase for cloning-based experiments. Traditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using 0.1-1 … game of thrones s07e01 ettv subtitle
T4 DNA Ligase Buffer (10X) - Thermo Fisher Scientific
Web- 400 uL 1M Tris pH 7.5 - 80 uL 0.5 EDTA - 400 uL 5M NaCl 3 Pipette 95 uL of annealing buffer to 48 wells (half a plate or 8 strip tubes) ... 0.2 µL NEB T4 DNA ligase, LC 40 µL NEB T4 DNA ligase WebNEBNext Quick Ligation Module Protocol (E6056) Protocol Mix the following components in a sterile microfuge tube: Incubate in a thermocycler for 15 minutes at 20°C with heated lid set to 30°C (or off). Purify DNA sample using AMPure ® XP or SPRIselect ® beads. WebJan 11, 2024 · For in situ RNA-DNA proximity ligation, nuclei were resuspended in 2 mL of proximity ligation mixture (4 U/µL T4 DNA ligase (NEB), 1× DNA ligase reaction buffer, 0.1% Triton X-100, 1 mg/mL BSA (NEB), 0.5 U/µL … game of thrones s06e09 budget