WebFeb 15, 2024 · Self-incompatibility in Prunus species is governed by a single locus consisting of two highly multi-allelic and tightly linked genes, one coding for an F-box protein—i.e., SFB in Prunus- controlling the pollen specificity and one coding for an S-RNase gene controlling the pistil specificity. Genotyping the allelic combination in a fruit … WebThe credibility of a pathogen detection assay is measured using specific parameters including repeatability, specificity, sensitivity, and reproducibility. The use of high-throughput sequencing (HTS) as a routine detection assay for viruses and viroids in citrus was previously evaluated and, in this study, the reproducibility and sensitivity of the HTS assay …
High-throughput sequencing - ScienceDirect
WebDec 2, 2024 · In all 3 cases a deep evaluation of the discrepant gene variant results and methodological approach allowed to confirm the NGS datum. Allelic drop-out (ADO) occurrence during polymerase chain or sequencing reaction was observed, mainly related to incorrect variant zygosity. ... Keywords: high-throughput sequencing, next generation … WebDesigned with the individual laboratory in mind, it is the first high-throughput desktop sequencer to offer exome, transcriptome, and whole genome sequencing in a compact package. It offers a variety of flow cell configurations including up … ridgwell windows and conservatories
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WebApr 3, 2024 · High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, we examine the critical role of sequencing library quality … WebThe NCI CCR Genomics Core in Building 37 provides NIH intramural investigators with a range of genomics technologies, available either as services delivered by the core, or as wal WebAdvances in both high-throughput sequencing technologies and bioinformatics have broadened the views on disease pathogenesis, helping to identify biomarkers and develop therapeutic strategies . A combination of WGCNA (weighted gene coexpression network analysis) and DEGs (differentially expressed genes) has been widely applied in … ridgways tea